![]() Repeatability was 7.66% ± 15.89 between triplicates for all antigens and samples, being lower in samples from malaria-exposed than malaria-naïve individuals. The overall mean of non-specific binding measured in 288 plasma samples was 32.83 to ± 44.81 median fluorescence intensity (MFI). The multiplex quantitative suspension assay demonstrated low non-specific signal and good estimates of precision and reproducibility between operators. Positive controls, negative controls, blanks and microspheres coated with bovine serum albumin were included in all assays. ![]() Another set of samples from 9 malaria-naïve and 10 malaria-exposed individuals were repetitively assayed during 22 consecutive days. A set of 288 human plasma samples from a malaria-endemic region were analysed twice by two different operators. A multiplex quantitative suspension assay to measure antigen-specific IgGs was developed and its precision (reproducibility and repeatability), dynamic range, limits of detection and quantification, and non-specific binding to different P. Studies of anti-malarial naturally acquired and vaccine immunity would benefit from a standard high-throughput immunoassay to measure multiple antibodies. Finding reliable IgG biomarkers of protection is complicated by a parasite proteome of over 5000 proteins, some with polymorphisms. Antibody responses to Plasmodium falciparum play a critical role in disease control.
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